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| Registros recuperados: 237 | |
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| Gutiérrez,Camila; Zenis,Javier; Legarraga,Paulette; Cabrera-Pardo,Jaime R.; García,Patricia; Bello-Toledo,Helia; Opazo-Capurro,Andrés; González-Rocha,Gerardo. |
| ABSTRACT Global dissemination of mcr-like genes represents a serious threat to public health since it jeopardizes the effectiveness of colistin, an antibiotic used as a last-resort treatment against highly antibiotic-resistant bacteria. In 2017, a mcr-1-positive isolate of Escherichia coli was found in Chile for the first time. Herein we report the genetic features of this strain (UCO-457) by whole-genome sequencing (WGS) and conjugation experiments. The UCO-457 strain belonged to ST4204 and carried a 285 kb IncI2-type plasmid containing the mcr-1 gene. Moreover, this plasmid was transferred by conjugation to an E. coli J53 strain at high frequency. The isolate harbored the cma, iroN, and iss virulence genes and did carry resistance genes to... |
| Tipo: Info:eu-repo/semantics/other |
Palavras-chave: Colistin-resistance; Mcr-1; Escherichia coli; Chile. |
| Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702019000300203 |
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| Campos,M.R.H.; André,M.C.D.P.B.; Borges,L.J.; Kipnis,A.; Pimenta,F.C.; Serafini,A.B.. |
| From February 2004 to March 2005, 140 samples of food handlers - hands and nostrils - (92), raw milk (24), and minas frescal cheese (24) were analyzed for the presence of Escherichia coli in a dairy processing plant of Goiás State. Forty-seven E. coli strains were obtained and compared by DNA macrorestriction patterns obtained from pulsed-field gel electrophoresis following XbaI restriction in order to investigate the possible sources of cheese contaminations. Based on PFGE genotyping, one strain isolated from food the hands of a handler and five strains isolated from raw milk were identical or closely related to six strains from cheese suggesting, in these cases, the probable source of E. coli contamination in cheeses. No strain isolated from the nostrils... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Escherichia coli; Pulsotypes; Minas frescal cheese; Raw milk; Food handlers. |
| Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352009000500025 |
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| Conceição,R.A.; Ludovico,M.S.; Andrade,C.G.T.J.; Yano,T.. |
| The adhesins of extraintestinal pathogenic Escherichia coli are essential for mediating direct interactions between the microbes and the host cell surfaces that they infect. Using fluorescence microscopy and gentamycin protection assays, we observed that 49 sepsis-associated E. coli (SEPEC) strains isolated from human adults adhered to and invaded Vero cells in the presence of D-mannose (100%). In addition, bacteria concentrations of approximately 2 x 10(7) CFU/mL were recovered from Vero cells following an invasion assay. Furthermore, PCR analysis of adhesin genes showed that 98.0% of these SEPEC strains tested positive for fimH, 69.4% for flu, 53.1% for csgA, 38.8% for mat, and 32.7% for iha. Analysis of the invasin genes showed that 16.3% of the SEPEC... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Adhesion; Invasiveness; Escherichia coli; Sepsis. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012000500007 |
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| Tiba,Monique Ribeiro; Moura,Claúdia de; Carazzolle,Marcelo Falsarella; Leite,Domingos da Silva. |
| Escherichia coli has been isolated frequently, showing flagellar antigens that are not recognized by any of the 53 antisera, provided by the most important reference center of E. coli, The International Escherichia and Klebsiella Center (WHO) of the Statens Serum Institute, Copenhagen, Denmark. The objective of this study was to characterize flagellar antigens of E. coli that express non-typeable H antigens. The methods used were serology, PCR-RFLP and DNA sequencing. This characterization was performed by gene amplification of the fliC (flagellin protein) by polymerase chain reaction in all 53 standards E.coli strains for the H antigens and 20 E. coli strains for which the H antigen was untypeable. The amplicons were digested by restriction enzymes, and... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Escherichia coli; Antigens; Bacterial; Polymerase chain reaction; Polymorphism; Restriction fragment length. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702011000200009 |
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| Mieszkin, Sophie; Caprais, Marie-paule; Le Mennec, Cecile; Le Goff, Manon; Edge, T. A.; Gourmelon, Michele. |
| Aims: The aim of this study was to identify the origin of faecal pollution impacting the Elorn estuary (Brittany, France) by applying microbial source tracking (MST) markers in both oysters and estuarine waters. Methods and Results: The MST markers used were as follows: (i) human-, ruminant- and pig-associated Bacteroidales markers by real-time PCR and (ii) human genogroup II and animal genogroup I of F-specific RNA bacteriophages (FRNAPH) by culture/genotyping and by direct real-time reverse-transcriptase PCR. The higher occurrence of the human genogroup II of F-specific RNA bacteriophages using a culture/genotyping method, and human-associated Bacteroidales marker by real-time PCR, allowed the identification of human faecal contamination as the... |
| Tipo: Text |
Palavras-chave: Escherichia coli; Estuarine and river waters; F-specific RNA bacteriophages; Host-associated Bacteroidales markers; Microbial source tracking; Shellfish. |
| Ano: 2013 |
URL: http://archimer.ifremer.fr/doc/00153/26426/25250.pdf |
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| Bastos,E.M.A.F; Galbiati,C; Loureiro,E.M; Scoaris,D.O. |
| The activity of 23 samples of ethanolic brown propolis, from the State of Mato Grosso, was investigated against Escherichia coli ATCC 25922. The values of physical and chemical parameters showed significant variation among samples. The percentage of dry extract ranged from 2.6 to 27.6%. The index of oxidation varied from 3 to 519 seconds. All samples showed the percentage of wax higher than the limit preconized by the legislation, with values varying from 3.4 to 74.6%. The quantification of phenolic and flavonoid compounds, responsible for antimicrobial activity, ranged from 0.1 to 5.0 (w/w) and 0.02 to 0.66 (w/w), respectively, being that the higher the index of phenolic compounds the larger the zones of inhibition. Antibacterial activity was observed in... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Abelha; Própolis; Atividade antibacteriana; Indicadores físico-químicos; Escherichia coli. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352011000500032 |
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| Santos-Filho,Sebastião David; Diniz,Claudia Leite; Carmo,Fernanda Santos do; Fonseca,Adenilson de Souza da; Bernardo-Filho,Mario. |
| The aim of this work was to study the influence of a walnut (Juglans regia) extract on the growth of Escherichia coli (E. coli) AB1157, on the plasmid DNA topology and on the labeling of blood constituents with technetium-99m (99mTc). An E. coli AB1157 culture, in stationary phase, was incubated with walnut and the growth of the culture was evaluated by optical density at 600 nm for 7 hours. Plasmid DNA samples were incubated with SnCl2 in presence or absence of walnut for 40 minutes, 0.8% agarose gel electrophoresis was performed, the gel was stained and the plasmid topological forms were visualized. Blood samples from Wistar rats were incubated with walnut extract and an assay of labeling of blood constituents with technetium-99m (99mTc) was performed.... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Escherichia coli; DNA; Electrophoresis; Stannous chloride; Technetium-99m; Juglans regia. |
| Ano: 2008 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000700026 |
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| Vaz,Michelle Rossana Ferreira; França,Ricardo Luiz Soares de; Andrade,Sirtys Santos Lessa de; Sousa Junior,Francisco Canindé de; Santos,Everaldo Silvino dos; Martins,Daniella Regina Arantes; Macedo,Gorete Ribeiro de. |
| With the advent of recombinant DNA technology, recombinant protein expression has become an important tool in the study of the structure, function and identification of new proteins, especially those with therapeutic functions. Escherichia coli has been the predominant prokaryote used in genetic engineering studies due to the abundance of information about its metabolism. Despite significant advances in molecular biology and immunology of infections, there are as yet no prophylactic drugs capable of preventing visceral leishmaniasis. It is therefore important to identify specific antigens in order to develop vaccines and diagnostic kits against this disease. The objective of this study was to evaluate the influence of culture medium on the production of... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Cultivation; Recombinant protein; Visceral leishmaniasis; Escherichia coli. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000400021 |
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| Srednik,M.E.T; Rumi,M.V.; Bentancor,A. |
| Escherichia coli es un marcador de contaminación fecal en alimentos. Dentro de la especie se encuentran patotipos de riesgo para la salud. Entre ellos, E. coli enteropatógeno y enterohemorrágico tienen la habilidad de causar la lesión de adherencia y esfacelación (A/E) en células intestinales. El estudio de ambos patotipos engloba aquellos más frecuentemente relacionados a diarrea infantil. El objetivo del presente trabajo fue evaluar la presencia de cepas A/E, particularmente las enteropatógenas que serían consideradas inocuas bajo la normativa de control actual. Se analizaron 98 muestras de carne molida compradas en carnicerías de Buenos Aires. La presencia de los patotipos se analizó mediante PCR de los genes (a) eae que codifica la proteína intimina,... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: AEEC; Escherichia coli; Carne molida; Calidad; Inocuidad. |
| Ano: 2013 |
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S1668-34982013000200005 |
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| Cossi,Marcus Vinícius Coutinho; Almeida,Michelle Vieira de; Dias,Mariane Rezende; Pinto,Paulo Sérgio de Arruda; Nero,Luís Augusto. |
| Sixty samples of chilled chicken carcasses submitted (30) and not submitted (30) to Brazilian inspection services were analyzed to investigate if inspected and non-inspected chilled carcasses represented different food safety risks in the region of Viçosa, MG, Brazil. The mean counts of indicator microorganisms (mesophilic aerobes, Enterobacteriaceae, total coliforms and Escherichia coli) of samples belonging to the inspected and non-inspected lots did not present significant differences (P>0.05). Also, no significant differences (P>0.05) were observed for the numbers of Salmonella spp. and E. coli (higher than 2log cfu g-1) between samples submitted or not to inspection. Statistical differences were observed between the two sample classes... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Inspection; Salmonella; Escherichia coli; Enterobacteriaceae; Hygiene indicators. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782012000900025 |
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| Derolez, Valerie; Serais, Ophelie; Caprais, Marie-paule; Le Saux, Jean-claude; Messiaen, Gregory. |
| Microbes regularly contaminate Thau lagoon shellfish. Between 1997 and 2007, 18% of the analyses carried out in dry weather recorded more than 230 Escherichia coli / 100 g of bivalves (Ifremer French microbial monitoring network), leading the administration to restrict the shellfish harvest area, including suspension of production. This study aims to determine, the contribution of seabirds, which roost at night on the Thau lagoon shellfish growing frames, to shellfish faecal pollution in dry weather.Data were collected in 2008 from both the drainage area (pollution input) and the lagoon (water quality and microbial monitoring, seabird counts) in dry weather. The data showed that, with no watershed input or lagoon resurgence, microbial contaminants were... |
| Tipo: Text |
Palavras-chave: Escherichia coli; Origine aviaire; Coquillage; Contamination fécale; Étang de Thau; Oiseau de mer; Bactériophage; Dépistage de la source microbienne; Temps sec; Escherichia coli; Avian source; Shellfish; Faecal contamination; Thau lagoon; Seabird; Bacteriophage; Microbial source tracking; Dry weather. |
| Ano: 2009 |
URL: http://archimer.ifremer.fr/doc/00025/13593/10659.pdf |
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| Espinosa,Ivette; Colas,M; Vichi,Joivier; Báez,M; Martínez,Siomara. |
| Respiratory infection is the most serious disease affecting poultry and causing great economic losses to the poultry industry worldwide. In avian host, several microorganisms of the genus Pasteurella (P. multocida, P. gallinarum and P. anatipestifer), Bordetella (B. avium) and Avibacterium paragallinarum) were involved in respiratory diseases complex. Ornithobacterium rhinotracheale is a recently discovered bacterium, of worldwide distribution in commercial poultry, in which it is associated to respiratory diseases and it is also found in wild birds. Airsacculitis and pneumonia are the most common symptoms of infection with O. rhinotracheale. Isolation and identification of pathogenic bacteria associated to respiratory diseases were carried out from a... |
| Tipo: Journal article |
Palavras-chave: Pasteurella multocida; Escherichia coli; Ornithobacterium rhinotracheale; Layer hens. |
| Ano: 2011 |
URL: http://scielo.sld.cu/scielo.php?script=sci_arttext&pid=S0253-570X2011000100006 |
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| Registros recuperados: 237 | |
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